PACT Antibody (PSH02-14)

Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705]

Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32705, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705]

Immunohistochemistry: PACT Antibody (PSH02-14) [NBP3-32705] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-PACT antibody (NBP3-32705) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32705) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: PACT Antibody (PSH02-14) [NBP3-32705]

Immunocytochemistry/ Immunofluorescence: PACT Antibody (PSH02-14) [NBP3-32705] - Immunocytochemistry analysis of 293T cells labeling PACT with Rabbit anti-PACT antibody (NBP3-32705) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PACT antibody (NBP3-32705) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.