PAF49 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19676

Novus Biologicals, part of Bio-Techne
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NBP2-19676
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PAF49 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Localization

Nucleus; nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAF49 Antibody

Western Blot: PAF49 Antibody [NBP2-19676]

Western Blot: PAF49 Antibody [NBP2-19676]

Western Blot: PAF49 Antibody [NBP2-19676] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PAF49 Antibody [NBP2-19676]

Immunocytochemistry/ Immunofluorescence: PAF49 Antibody [NBP2-19676]

Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PAF49 Antibody [NBP2-19676]

Immunohistochemistry-Paraffin: PAF49 Antibody [NBP2-19676]

Immunohistochemistry-Paraffin: PAF49 Antibody [NBP2-19676] - Paraffin-embedded human ovarian cancer. PAF49 antibody diluted at 1:500.
View 1 more images

Applications for PAF49 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAF49

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

Alternate Names

A34.5, antisense to ERCC 1, Antisense to ERCC-1 protein, ASE-1ASE1, CASTMGC118851, CD3 epsilon associated protein, CD3e antigen, epsilon polypeptide associated protein, CD3e molecule, epsilon associated protein, CD3E-associated protein, CD3-epsilon-associated protein, DNA-directed RNA polymerase I subunit RPA34, PAF49RNA polymerase I-associated factor PAF49

Gene Symbol

CD3EAP

UniProt

O15446 (Human)

Additional PAF49 Products

Product Documents for PAF49 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAF49 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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