PAG1 Antibody (SR2238)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22384

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22384-100ul
NBP3-22384-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2238

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAG1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PAG1 (Uniprot #: Q9NWQ8)

Specificity

Detects endogenous levels of total PAG1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAG1 Antibody (SR2238)

Western Blot: PAG1 Antibody (SR2238) [NBP3-22384] -

Western Blot: PAG1 Antibody (SR2238) [NBP3-22384] - Western blot analysis using NBP3-22384 in Raji cell lysate.

Applications for PAG1 Antibody (SR2238)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAG1

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Long Name

Phosphoprotein Associated with Glycosphingolipid Microdomains 1

Alternate Names

CBP

Gene Symbol

PAG1

Additional PAG1 Products

Product Documents for PAG1 Antibody (SR2238)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PAG1 Antibody (SR2238)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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