PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16930
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1T7A5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S144 of human PAK1/2/3 (Q13153). YMSFT
Modification
p Ser 144, p Ser 141, p Ser 139
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)
Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Applications for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Application Notes
Modifications: PAK1 - p Ser 144, PAK2 - p Ser 141, PAK3 - p Ser 139
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PAK1/2/3
Alternate Names
alpha-PAK, EC 2.7.11, EC 2.7.11.1, MGC130000, MGC130001, p21 protein (Cdc42/Rac)-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, p65-PAK, PAK-1, PAKalpha, serine/threonine-protein kinase PAK 1, STE20 homolog, yeast
Gene Symbol
PAK1
Additional PAK1/2/3 Products
Product Documents for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)
Product Specific Notices for PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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