PAK3 Antibody (0K1I5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16173

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16173-100ul
NBP3-16173-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0K1I5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PAK3 Antibodies »

Product Summary for PAK3 Antibody (0K1I5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAK3 (O75914). MSDGLDNEEKPPAPPLRMNSNNRDSSALNHSSKPLPMAPEEKNKKARLRSIFPGGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTPDLYGSQM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PAK3 Antibody (0K1I5)

Western Blot: PAK3 Antibody (0K1I5) [NBP3-16173]

Western Blot: PAK3 Antibody (0K1I5) [NBP3-16173]

Western Blot: PAK3 Antibody (0K1I5) [NBP3-16173] - Western blot analysis of extracts of various cell lines, using PAK3 Rabbit mAb (NBP3-16173) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: PAK3 Antibody (0K1I5) [NBP3-16173]

Immunohistochemistry-Paraffin: PAK3 Antibody (0K1I5) [NBP3-16173]

Immunohistochemistry-Paraffin: PAK3 Antibody (0K1I5) [NBP3-16173] - Immunohistochemistry of paraffin-embedded mouse brain using PAK3 Rabbit mAb (NBP3-16173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PAK3 Antibody (0K1I5) [NBP3-16173]

Immunohistochemistry-Paraffin: PAK3 Antibody (0K1I5) [NBP3-16173]

Immunohistochemistry-Paraffin: PAK3 Antibody (0K1I5) [NBP3-16173] - Immunohistochemistry of paraffin-embedded rat brain using PAK3 Rabbit mAb (NBP3-16173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for PAK3 Antibody (0K1I5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PAK3

p21-activated kinase 3 (PAK3) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, group I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). All six PAKs play an important role in diverse cellular processes, including cytoskeletal dynamics, growth/apoptotic signal transduction through MAP kinases, and regulation of transcription factors (4). PAK3 as well as PAK1 are mostly expressed in the nervous system and particularly in the hippocampus, in which they show a diffuse distribution throughout the soma and proximal dendrites (5). Mutations in PAK3 have been linked to nonsyndromic X-linked mental retardation (6).

Long Name

p21-activated Kinase 3

Alternate Names

beta-PAK, MRX30, MRX47, OPHN3

Gene Symbol

PAK3

Additional PAK3 Products

Product Documents for PAK3 Antibody (0K1I5)

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Certificate of Analysis

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Product Specific Notices for PAK3 Antibody (0K1I5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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