PAK6 [p Ser165] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23266
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PAK6 (Ser-165) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6. [UniProt# Q9NQU5]
Modification
p Ser165
Specificity
This antibody was cross-adsorbed to unphosphorylated PAK6 (Ser-165) peptide then affinity purified using phospho-PAK6 (Ser-165) peptide. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-PAK6 (Ser-165) peptide and also by treatment with lambda phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for PAK6 [p Ser165] Antibody
![PAK6 [p Ser165] Antibody](https://resources.bio-techne.com/images/products/nbp3-23266_rabbit-pak6-p-ser165-pab-19920231856915.jpg "Western Blot: PAK6 [p Ser165] Antibody [NBP3-23266] -")
Western Blot: PAK6 [p Ser165] Antibody [NBP3-23266] -
Western Blot: PAK6 [p Ser165] Antibody [NBP3-23266] - Western blots of human PAK6 recombinant protein phosphorylated by ERK2. The blot was exposed to lambda phosphatase (lanes 2 & 6) then probed with anti-PAK6 (N-terminal) (lanes 1-4) or anti-PAK6 (Ser-165) phospho-specific (lanes 5-8). The antibodies were used in the presence of unrelated (lane 3) and PAK6 (N-terminal) (lane 4) peptide or PAK6 (Ser-165) (lane 7) and unrelated phospho-serine (lane 8) peptides, respectively.Applications for PAK6 [p Ser165] Antibody
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: PAK6
Long Name
p21/Cdc42/Rac1-activated Kinase 6
Alternate Names
EC 2.7.11, p21 protein (Cdc42/Rac)-activated kinase 6, p21(CDKN1A)-activated kinase 6, p21-activated kinase 6, p21-activated protein kinase 6, PAK-5, PAK5EC 2.7.11.1, PAK-6, serine/threonine-protein kinase PAK 6
Gene Symbol
PAK6
Additional PAK6 Products
Product Documents for PAK6 [p Ser165] Antibody
Product Specific Notices for PAK6 [p Ser165] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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