Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PALB2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human partner and localizer of BRCA2 using the numbering given in entry NP_078951.2 (GeneID 79728).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PALB2 Antibody

Western Blot: PALB2 Antibody [NB100-60440]

Western Blot: PALB2 Antibody [NB100-60440]

Western Blot: PALB2 Antibody [NB100-60440] - Whole cell lysate (50 ug) from HeLa and cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PALB2 antibody NB100-60440 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: PALB2 Antibody [NB100-60440]

Immunohistochemistry-Paraffin: PALB2 Antibody [NB100-60440]

Immunohistochemistry-Paraffin: PALB2 Antibody [NB100-60440] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PALB2 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
Immunoprecipitation: PALB2 Antibody [NB100-60440]

Immunoprecipitation: PALB2 Antibody [NB100-60440]

Immunoprecipitation: PALB2 Antibody [NB100-60440] - Detection of human PALB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PALB2 antibody NB100-60440 used for IP at 6 ug per reaction. PALB2 was also immunoprecipitated by rabbit anti-PALB2 antibody NB100-60439. For blotting immunoprecipitated PALB2, NB100-60440 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PALB2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PALB2

PALB2 (partner and localizer of BRCA2) interacts with BRCA2 and promotes BRCA2 localization and stability. PALB2 is also known as FANCN. Bi-allelic mutations in PALB2 result in Fanconi anemia disorder and breast cancer susceptibility. At the cellular level, PALB2 is important to the DNA damage response and chromosome stability and is essential to BRCA2 function.

Long Name

Partner and Localizer of BRCA2

Alternate Names

FANCN

Entrez Gene IDs

79728 (Human)

Gene Symbol

PALB2

UniProt

NP_078951.2 (Human)

Additional PALB2 Products

Product Documents for PALB2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PALB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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