pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25605
Recombinant Monoclonal Antibody
![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249422634.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Drosophila
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1279
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr410 of human pan PKC zeta. The exact sequence is proprietary.
Modification
p Thr 410
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940840.jpg "Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Immunocytochemistry/Immunofluorescence: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - PKC (pan) (zeta phospho Thr410) antibody [HL1279] detects PKC (pan) (zeta phospho Thr410) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKC (pan) (zeta phospho Thr410) stained by PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024941594.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249342742.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -
Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1279] (NBP3-25605) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249415946.jpg "Immunohistochemistry-Paraffin: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-212024940812.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
![pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-25605_rabbit-pan-pkc-p-thr-410-mab-hl1279-azide-and-bsa-free-2120249383155.jpg "Western Blot: pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free [NBP3-25605] -")
Applications for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: pan PKC
Alternate Names
AAG6;PKCA;PKCalpha;PKC-alpha;PKCI+/-;PRKACA;PRKCA;Protein kinase C;Protein kinase C alpha type
Gene Symbol
PRKCA
Additional pan PKC Products
Product Documents for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
Product Specific Notices for pan PKC [p Thr410] Antibody (HL1279) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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