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Pancreatic trypsin inhibitor Antibody (18) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23588

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 18

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Aprotinin from bovine lung.

Epitope

The epitope of Clone 18 is different from the epitopes of Clone 21 and Clone 35

Specificity

Clone 18 binds bovine aprotinin immobilized on a solid phase or free in solution.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Pancreatic trypsin inhibitor Antibody (18) - BSA Free

ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588]

ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588]

ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588] - The calibration curve of a sandwich assay for Aprotinin (low sensitivity) using NBP2-23590 as the capture antibody and NBP2-23587 as the biotinylated detection antibody.
ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588]

ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588]

ELISA: Pancreatic trypsin inhibitor Antibody (18) - BSA Free [NBP2-23588] - The calibration curve of a sandwich assay for Aprotinin (high sensitivity) using NBP2-23589 as the capture antibody and NBP2-23587 as the biotinylated detection antibody.

Applications for Pancreatic trypsin inhibitor Antibody (18) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-23588 (as biotinylated detection antibody e.g. at 1 ug/mL) forms an antibody pair with NBP2-23589 (as capture antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 ug/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. NBP2-23588 also forms an antibody pair with NBP2-23590 (as capture antibody) for sandwich ELISA of aprotinin at sample concentrations of 4-250 ug/mL. Both urine and plasma samples diluted 1/1000 can be assayed against standards in buffer. PBS-buffers are unsuitable for these assays and 0.2 M glycine sodium buffer, pH 8.8, is recommended. WB: NBP2-23588 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Pancreatic trypsin inhibitor

Aprotinin, also called bovine pancreatic trypsin inhibitor, is a single chain globular Kunitz-type serine proteinase inhibitor found in bovine pancreas, parotid gland and lung. It forms stable, enzymatically inactive complexes with trypsin, chymotrypsin, plasmin and kallikrein. Aprotinin has been used therapeutically as an antifibrinolytic agent under the name Trasylol (Bayer) to reduce blood loss during cardiopulmonary bypass surgery but this use has been associated with an increased frequency of acute renal injury

Alternate Names

Aprotinin, Basic protease inhibitor, BPI, BPTI, PTI, PTI pancreatic trypsin inhibitor

Gene Symbol

PTI

Additional Pancreatic trypsin inhibitor Products

Product Documents for Pancreatic trypsin inhibitor Antibody (18) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pancreatic trypsin inhibitor Antibody (18) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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