Pancreatic trypsin inhibitor Antibody (21) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23589
![ELISA: Pancreatic trypsin inhibitor Antibody (21) - BSA Free [NBP2-23589]](https://resources.bio-techne.com/images/products/Pancreatic-trypsin-inhibitor-Antibody-21-BSA-Free-ELISA-NBP2-23589-img0001.jpg "ELISA: Pancreatic trypsin inhibitor Antibody (21) - BSA Free [NBP2-23589]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Bovine
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 21
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Aprotinin from bovine lung
Epitope
The epitope of Clone 21 is different from the epitopes of Clone 18 and Clone 35
Specificity
Clone 21 binds bovine aprotinin immobilized on a solid phase or free in solution.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Pancreatic trypsin inhibitor Antibody (21) - BSA Free
ELISA: Pancreatic trypsin inhibitor Antibody (21) - BSA Free [NBP2-23589]
ELISA: Pancreatic trypsin inhibitor Antibody (21) - BSA Free [NBP2-23589] - The calibration curve of a sandwich assay for Aprotinin (high sensitivity) using NBP2-23589 as the capture antibody and NBP2-23587 as the biotinylated detection antibody.Applications for Pancreatic trypsin inhibitor Antibody (21) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP2-23589 (as capture antibody coated at 2 ug/mL) forms an antibody pair with NBP2-23588 (as biotinylated detection antibody) for high-sensitivity sandwich ELISA of aprotinin at sample concentrations of 0.6-20 ug/mL. Aprotinin in urine diluted 1/1000 can be assayed against standards in buffer. For assay in plasma it is recommended to add aprotinin-free control plasma to the standards to match the plasma sample dilution. PBS-based buffers are unsuitable for this assay and 0.2 M glycine sodium buffer, pH 8.8, is recommended for washing and dilution buffers.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Pancreatic trypsin inhibitor
Alternate Names
Aprotinin, Basic protease inhibitor, BPI, BPTI, PTI, PTI pancreatic trypsin inhibitor
Gene Symbol
PTI
Additional Pancreatic trypsin inhibitor Products
Product Documents for Pancreatic trypsin inhibitor Antibody (21) - BSA Free
Product Specific Notices for Pancreatic trypsin inhibitor Antibody (21) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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