Skip to main content

PANK2 Antibody (OTI3H9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73241

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-73241
Conjugate
Catalog #

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3H9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human PANK2(NP_705902) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

59.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PANK2 Antibody (OTI3H9) - Azide and BSA Free

Western Blot: PANK2 Antibody (OTI3H9)Azide and BSA Free [NBP2-73241]

Western Blot: PANK2 Antibody (OTI3H9)Azide and BSA Free [NBP2-73241]

Western Blot: PANK2 Antibody (OTI3H9) - Azide and BSA Free [NBP2-73241] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PANK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PANK2.
Immunocytochemistry/ Immunofluorescence: PANK2 Antibody (OTI3H9) - Azide and BSA Free [NBP2-73241]

Immunocytochemistry/ Immunofluorescence: PANK2 Antibody (OTI3H9) - Azide and BSA Free [NBP2-73241]

Immunocytochemistry/Immunofluorescence: PANK2 Antibody (OTI3H9) - Azide and BSA Free [NBP2-73241] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PANK2.
Immunohistochemistry: PANK2 Antibody (OTI3H9) - Azide and BSA Free [NBP2-73241]

Immunohistochemistry: PANK2 Antibody (OTI3H9) - Azide and BSA Free [NBP2-73241]

Immunohistochemistry: PANK2 Antibody (OTI3H9) - Azide and BSA Free [NBP2-73241] - Staining of paraffin-embedded Human liver tissue using anti-PANK2 mouse monoclonal antibody.

Applications for PANK2 Antibody (OTI3H9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PANK2

PANK2 encodes a protein belonging to the pantothenate kinase family and is the only member of that family to be expressed in mitochondria. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by acyl CoA species. Mutations in this gene are associated with HARP syndrome and pantothenate kinase-associated neurodegeneration (PKAN), formerly Hallervorden-Spatz syndrome. Alternative splicing, involving the use of alternate first exons, results in multiple transcripts encoding different isoforms.

Long Name

Pantothenate Kinase 2

Alternate Names

FLJ11729, HPANK2, NBIA1, PKAN

Gene Symbol

PANK2

Additional PANK2 Products

Product Documents for PANK2 Antibody (OTI3H9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PANK2 Antibody (OTI3H9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...