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PAPD1 Antibody (1D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43686

Novus Biologicals, part of Bio-Techne
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NBP2-43686

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1D3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to human PAPD1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAPD1 Antibody (1D3)

Immunocytochemistry/ Immunofluorescence: PAPD1 Antibody (1D3) [NBP2-43686]

Immunocytochemistry/ Immunofluorescence: PAPD1 Antibody (1D3) [NBP2-43686]

Immunocytochemistry/Immunofluorescence: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of methanol-fixed HeLa, using mtPAP antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: PAPD1 Antibody (1D3) [NBP2-43686]

Immunohistochemistry-Paraffin: PAPD1 Antibody (1D3) [NBP2-43686]

Immunohistochemistry-Paraffin: PAPD1 Antibody (1D3) [NBP2-43686] - Analysis of Hepatoma, using mtPAP antibody at 1:200 dilution.
PAPD1 Antibody (1D3)

Western Blot: PAPD1 Antibody (1D3) [NBP2-43686] -

Western Blot: PAPD1 Antibody (1D3) [NBP2-43686] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with mtPAP antibody [1D3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for PAPD1 Antibody (1D3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH7), 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAPD1

Polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. Plays a role in replication-dependent histone mRNA degradation. May be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated. Might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA

Alternate Names

EC 2.7.7.19, FLJ10486, mitochondrial poly(A) polymerase, mtPAP, PAP, PAP associated domain containing 1, PAP-associated domain-containing protein 1, PAPD1SPAX4, poly(A) RNA polymerase, mitochondrial, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, TUTase 1

Gene Symbol

MTPAP

Additional PAPD1 Products

Product Documents for PAPD1 Antibody (1D3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAPD1 Antibody (1D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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