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PAPD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19690

Novus Biologicals, part of Bio-Techne
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NBP2-19690

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PAPD1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAPD1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PAPD1 Antibody

Western Blot: PAPD1 Antibody [NBP2-19690]

Western Blot: PAPD1 Antibody [NBP2-19690]

Western Blot: PAPD1 Antibody [NBP2-19690] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: PAPD1 Antibody [NBP2-19690]

Immunocytochemistry/ Immunofluorescence: PAPD1 Antibody [NBP2-19690]

Immunocytochemistry/Immunofluorescence: PAPD1 Antibody [NBP2-19690] - Analysis of methanol-fixed HeLa, using Mt PAP(GTX118738) antibody at 1:200 dilution.

Applications for PAPD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000
Application Notes
IP-Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAPD1

The protein encoded by this gene is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation.

Alternate Names

EC 2.7.7.19, FLJ10486, mitochondrial poly(A) polymerase, mtPAP, PAP, PAP associated domain containing 1, PAP-associated domain-containing protein 1, PAPD1SPAX4, poly(A) RNA polymerase, mitochondrial, Polynucleotide adenylyltransferase, Terminal uridylyltransferase 1, TUTase 1

Gene Symbol

MTPAP

UniProt

Additional PAPD1 Products

Product Documents for PAPD1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PAPD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.