PARD3/Par3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11253

Novus Biologicals, part of Bio-Techne
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NBP2-11253
NBP2-11253-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all PARD3/Par3 Antibodies »

Product Summary for PARD3/Par3 Antibody

Immunogen

Synthetic peptide from amino acids 31-342 corresponding to rat PAR2 protein from the following sequence, was selected from the unique region of the mouse PAR3 protein, peptide was post-synthetically modified to achieve highest antigenicity before used for coupling to KLH using heterobifunctional cross linker for immunogen preparation.

Epitope

KPTLTIKSFNGGPQNTFEEFPLSDIE

Reactivity Notes

Mouse

Specificity

This antibody detects a band of approximately 48kDa PAR3 protein in samples. The antibody also labels a 58kDa bands in PAR3 samples, probably represents either an uncharacterized variant of PAR3 or a non-specific protein. Further experiments are needed to address these possibilities

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARD3/Par3 Antibody

Western Blot: PARD3/Par3 Antibody [NBP2-11253]

Western Blot: PARD3/Par3 Antibody [NBP2-11253]

Western Blot: PARD3 Antibody [NBP2-11253] - using Sample and antibody. Antibody was diluted 1:500 inDilution Buffer. MW of PAR3 is 48kDa.

Applications for PARD3/Par3 Antibody

Application
Recommended Usage

Western Blot

1:250
Application Notes
Antibodies to PAR3 label approximately 48kDa protein in Western blot positive control and several other tissues.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARD3/Par3

Serine proteases are well known as enzymes involved in digestion of dietary proteins, blood coagulation, and homeostasis. A new role of serine proteases as signal molecule acting via protease-activated receptors (PARs) was recently uncovered. Important effects of PAR activation on leukocyte motility, cytokine production, adhesion molecule expression, and a variety of other physiological or pathophysiological functions have been described in vitro and in vivo. PAR activation is also involved in a variety of diseases conditions including neuroinflammatory and neurodegenerative process of skin, joints, and airway inflammation. Additionally Par activation is involved in innate and adaptive immunity (1). PAR receptors belong to the 7TMD family members and are expressed by 4 distinct genes (PAR1-4). PAR receptors and purinergic receptors (P2RY12 ) play important role in homostasis and thrombosis. Par3knockout mice showed nearly complete protection against carotid artery thrombosis caused by low FeCl(3) injury. Par3 and Par4 heterozygous mouse platelets showed partially decreased responses to thrombin, resembling those in PAR1 antagonist-treated human platelets (2). PAR3 is a thrombin receptor that is expressed in mouse megakaryocytes and is responsible for normal thrombin responses in mouse platelets (3).

Long Name

Par-3 Partitioning Defective 3 Homolog

Alternate Names

ASIP, Baz, Bazooka, PAR3, PAR3A, SE2-5L16, SE2-5LT1, SE2-5T2

Gene Symbol

PARD3

Additional PARD3/Par3 Products

Product Documents for PARD3/Par3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PARD3/Par3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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