Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PARG1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1211 and 1261 of human PTPL1-associated RhoGAP 1 (NP_004806.3).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PARG1 Antibody

Western Blot: PARG1 Antibody [NBP1-05989]

Western Blot: PARG1 Antibody [NBP1-05989]

Western Blot: PARG1 Antibody [NBP1-05989] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. PARG1 was also immunoprecipitated by rabbit anti-PARG1 antibody NBP1-05988.

Applications for PARG1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
ICC/IF, IHC reactivity reported in scientific literature (PMID: 23008150). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PARG1

PARG1 (PTPL1-associated RhoGAP protein 1) is a GTPase-activating protein (GAP) for Rho and Rap2. PARG1 has recently been identified as a candidate tumor suppressor in mantle-cell lymphoma. Alternative names for PARG1 include Rho GTPase-activating protein 29 and ARHGAP29.

Alternate Names

PARG1PTPL1-associated RhoGAP 1 (PARG1), PTPL1-associated RhoGAP protein 1, Rho GTPase activating protein 29, rho GTPase-activating protein 29, Rho-type GTPase-activating protein 29, RP11-255E17.1

Entrez Gene IDs

9411 (Human); 214137 (Mouse)

Gene Symbol

ARHGAP29

UniProt

Q52LW3 (Human)

Additional PARG1 Products

Product Documents for PARG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PARG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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