Parkin Antibody (PRK8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29838

Novus Biologicals, part of Bio-Techne
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NBP2-29838
NBP2-29838-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PRK8

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Parkin Antibodies »

Product Specifications

Immunogen

Full length recombinant human Parkin. Epitope has been mapped to the second ring domain (aa 399-465).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

Parkin52kDa.May be observed as a doublet at 52 and 46kDa weight may be detected.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Relevant Entrez Gene Numbers: NM_004562

Scientific Data Images for Parkin Antibody (PRK8)

Western Blot: Parkin Antibody (PRK8) [NBP2-29838]

Western Blot: Parkin Antibody (PRK8) [NBP2-29838]

Western Blot: Parkin Antibody (PRK8) [NBP2-29838] - HEK293 cell lysate was probed with anti- Parkin, clone PRK8 (1:2000 dilution). Arrow indicates Parkin (~52kDa).

Applications for Parkin Antibody (PRK8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NBP2-29838 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites, containing 30% glycerol.

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Parkin

Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.

Long Name

Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1

Alternate Names

AR-JP, PARK2, PDJ, PRKN

Gene Symbol

PRKN

UniProt

O60260 (Human)

Additional Parkin Products

Product Documents for Parkin Antibody (PRK8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Parkin Antibody (PRK8)

Relevant Entrez Gene Numbers: NM_004562

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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