Parkin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1540

Novus Biologicals, part of Bio-Techne
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NB600-1540
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Parkin Antibodies »

Product Specifications

Immunogen

Synthetic peptide: (C)EQARWEAAS KETIK conjugated to KLH, corresponding to amino acids 399-412 of Human Parkin.

Localization

Cytoplasmic

Specificity

NB600-1540 reacts with recombinant wildtype Parkin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Parkin Antibody

Western Blot: Parkin Antibody [NB600-1540]

Western Blot: Parkin Antibody [NB600-1540]

Western Blot: Parkin Antibody [NB600-1540] - Western blot of HEK293t cell lysate expressing FLAG-tagged recombinant human Parkin detected using NB600-1540 at various dilutions (1:2,000, B-1:5,000, C-1:10,000). ECL exposure 1min. FLAG-tagged Parkin courtesy of Dr P.A. Robinson, University of Leeds, UK).

Applications for Parkin Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
WB: 1/1000 - 1/10000. Predicted molecular weight: 52 kDa. Notes: Dilute to working strength with 50mM TrisHCl buffer (pH 7.6) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit antibody is to be used).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Parkin

Parkinson's disease is a common neurodegenerative disease with complex clinical features. Mutations in the gene, Parkin (PARK2), appear to be responsible for the pathogenesis of autosomal recessive juvenile Parkinsonism. Parkin plays a role in the ubiquitin-mediated proteolytic pathway by removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of Parkin. Parkin may protect neurons against alpha synuclein toxicity, proteasomal dysfunction, gpr37 accumulation, and kainate-induced excitotoxicity. It may play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Parkin also regulates cyclin e during neuronal apoptosis and may represent a tumor suppressor gene.

Long Name

Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1

Alternate Names

AR-JP, PARK2, PDJ, PRKN

Entrez Gene IDs

5071 (Human)

Gene Symbol

PRKN

Additional Parkin Products

Product Documents for Parkin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Parkin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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