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PARP2 Antibody - Unpurified

Novus Biologicals, part of Bio-Techne | Catalog # NB100-185

Novus Biologicals, part of Bio-Techne
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NB100-185
NB100-185SS

Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Unpurified

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Residues 43-59 [QRQESKKMPVAGGKANK] of the 62 kDa human PARP-2 protein.

Localization

Nucleus

Specificity

N-terminus of human PARP 2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PARP2 Antibody - Unpurified

Western Blot: PARP2 AntibodyUnpurified [NB100-185]

Western Blot: PARP2 AntibodyUnpurified [NB100-185]

Western Blot: PARP2 Antibody [NB100-185] - Detection of PARP2 in NIH/3T3 cell lysate with polyclonal PARP2 antibody at a dilution of 1:250
Western Blot: PARP2 AntibodyUnpurified [NB100-185]

Western Blot: PARP2 AntibodyUnpurified [NB100-185]

Western Blot: PARP2 Antibody [NB100-185] - Western blot of analysis of PARP2 in 3T3 cell lysate using NB100-185 at 1:250 dilution.
PARP2 Antibody - Unpurified

Western Blot: PARP2 Antibody [NB100-185] - 

Western Blot: PARP2 Antibody [NB100-185] - Activation of signaling pathways by dandelion root extract. Western blots of proteins involved in programmed cell death and cell survival and inflammation. The protein levels, including ULK1 (NBP2-24738), Bcl-2 related protein A1 (NBP1-76715), and PARP2 (NB100-185) corresponded to the mRNA levels obtained during the gene expression analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27564258/) licensed under a CC-BY license.

Applications for PARP2 Antibody - Unpurified

Application
Recommended Usage

Western Blot

1:250-1:500
Application Notes
This antibody can be used for western blot, where the predicted molecular weight is 66 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

PBS

Format

Unpurified

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PARP2

Poly(ADP-ribose) polymerase 1, (PARP-1) and poly(ADP-ribose) polymerase 2 (PARP-2) catalyze the formation of poly(ADP-ribose) polymers in a DNA-dependent manner. PARP-2 displays automodification properties similar to PARP-1. These act by poly(ADP-ribosylation), which is a post-translational modification of nuclear proteins in response to DNA damage that activates the base excision repair machinery.

Long Name

Poly [ADP-ribose] polymerase 2

Alternate Names

ADPRT-2, ADPRT2, ADPRTL2, ARTD2, EC 2.4.2.30, hPARP-2, pADPRT-2, PARP-2

Entrez Gene IDs

10038 (Human)

Gene Symbol

PARP2

Additional PARP2 Products

Product Documents for PARP2 Antibody - Unpurified

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PARP2 Antibody - Unpurified

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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