PARP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2556

Novus Biologicals, part of Bio-Techne
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NB100-2556
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PARP2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LDLFEVEKDGEKE corresponding to internal region according to NP_005475.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PARP2 Antibody

Western Blot: PARP2 Antibody [NB100-2556]

Western Blot: PARP2 Antibody [NB100-2556]

Western Blot: PARP2 Antibody [NB100-2556] - (1ug/ml) staining of Human Spleen Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556]

Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556]

Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556] - (5ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556]

Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556]

Immunohistochemistry-Paraffin: PARP2 Antibody [NB100-2556] - (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PARP2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human spleen lysates (calculated MW of 60.8 kDa band according to NP_005475.1).
IHC: Paraffin embedded Human Skin and Adrenal Gland.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PARP2

This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Long Name

Poly [ADP-ribose] polymerase 2

Alternate Names

ADPRT-2, ADPRT2, ADPRTL2, ARTD2, EC 2.4.2.30, hPARP-2, pADPRT-2, PARP-2

Entrez Gene IDs

10038 (Human)

Gene Symbol

PARP2

UniProt

NP_005475.1 (Human)

Additional PARP2 Products

Product Documents for PARP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PARP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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