PASK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83037

Novus Biologicals, part of Bio-Techne
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NBP1-83037
NBP1-83037-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PASK Antibodies »

Product Summary for PASK Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AQEVDVNSFSWNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPDVGSLQEQGSCVLDDRELLLLTGTCVDLGQGRRFRESCVGHDPTEPLEVCLVSSEH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PASK Antibody

Western Blot: PASK Antibody [NBP1-83037]

Western Blot: PASK Antibody [NBP1-83037]

Western Blot: PASK Antibody [NBP1-83037] - Analysis in control (vector only transfected HEK293T lysate) and PASK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PASK Antibody [NBP1-83037]

Immunocytochemistry/ Immunofluorescence: PASK Antibody [NBP1-83037]

Immunocytochemistry/Immunofluorescence: PASK Antibody [NBP1-83037] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: PASK Antibody [NBP1-83037]

Immunohistochemistry-Paraffin: PASK Antibody [NBP1-83037]

Immunohistochemistry-Paraffin: PASK Antibody [NBP1-83037] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Applications for PASK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PASK

PASKIN (PAS-Kinase) is thought to regulate protein synthesis in cells. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.

Alternate Names

DKFZp434O051, EC 2.7.11.1, hPASK, KIAA0135DKFZp686P2031, PAS domain containing serine/threonine kinase, PAS-kinase, PASKINPAS domain-containing serine/threonine-protein kinase, PAS-serine/threonine kinase, STK37

Gene Symbol

PASK

Additional PASK Products

Product Documents for PASK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PASK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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