Pax2 Antibody (JJ082-08)

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849]

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of Pax2 on human kidney tissue lysates with Rabbit anti-Pax2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849]

Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-Pax2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849]

Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849] - Western blot analysis of Pax2 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat