Pax3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32381

Novus Biologicals, part of Bio-Techne
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NBP2-32381
NBP2-32381-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pax3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PHQPQTDYALSPLTGGLEPTTTVSASCSQRLDHMKSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pax3 Antibody

Immunocytochemistry/ Immunofluorescence: Pax3 Antibody [NBP2-32381]

Immunocytochemistry/ Immunofluorescence: Pax3 Antibody [NBP2-32381]

Immunocytochemistry/Immunofluorescence: Pax3 Antibody [NBP2-32381] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381]

Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381]

Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381] - Staining of human testis shows moderate positivity in nucleoli in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381]

Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381]

Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381] - Staining of human prostate shows no positivity in nucleoli in glandular cells as expected.
View 1 more images

Applications for Pax3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax3

PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These proteins play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.

Long Name

Paired Box Gene 3 [Waardenburg Syndrome 1]

Alternate Names

HUP2, WS1

Gene Symbol

PAX3

Additional Pax3 Products

Product Documents for Pax3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pax3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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