Pax6 Antibody (GT9412)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13636

Novus Biologicals, part of Bio-Techne
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NBP3-13636
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT9412

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pax6 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pax6. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Pax6 Antibody (GT9412)

Western Blot: Pax6 Antibody (GT9412) [NBP3-13636]

Western Blot: Pax6 Antibody (GT9412) [NBP3-13636]

Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636]

Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636]

Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636]

Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636]

Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for Pax6 Antibody (GT9412)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pax6

PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters® anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.

Long Name

Paired Box Gene 6

Alternate Names

keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6

Gene Symbol

PAX6

Additional Pax6 Products

Product Documents for Pax6 Antibody (GT9412)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pax6 Antibody (GT9412)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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