Pax6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-61654
Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Human (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunohistochemistry-Frozen (Negative), Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-REEKLRNQRRQASN corresponding to internal region according to NP_001595.2.
Epitope
REEKLRNQRRQASN
Specificity
This antibody is expected to recognise both reported isoforms (NP_000271.1 and NP_001595.2).
Marker
Neural Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Pax6 Antibody
![Western Blot: Pax6 Antibody [NB100-61654]](https://resources.bio-techne.com/images/products/Pax6-Antibody-Western-Blot-NB100-61654-img0002.jpg "Western Blot: Pax6 Antibody [NB100-61654]")
Western Blot: Pax6 Antibody [NB100-61654]
Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Flow Cytometry: Pax6 Antibody [NB100-61654]](https://resources.bio-techne.com/images/products/Pax6-Antibody-Flow-Cytometry-NB100-61654-img0003.jpg "Flow Cytometry: Pax6 Antibody [NB100-61654]")
Flow Cytometry: Pax6 Antibody [NB100-61654]
Flow Cytometry: Pax6 Antibody [NB100-61654] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Western Blot: Pax6 Antibody [NB100-61654]](https://resources.bio-techne.com/images/products/Pax6-Antibody-Western-Blot-NB100-61654-img0001.jpg "Western Blot: Pax6 Antibody [NB100-61654]")
Western Blot: Pax6 Antibody [NB100-61654]
Western Blot: Pax6 Antibody [NB100-61654] - (0.3ug/ml) staining of U251 (A), (0.03ug/ml) nuclear K562 (B) and (1ug/ml) nuclear HeLa (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Pax6 Antibody
Application
Recommended Usage
Flow Cytometry
10ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.03 - 1.0 ug/ml
Application Notes
Western blot: Approx 26kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8kDa according to Human NP_001355859.1 and 31.8 according to Mouse NP_001297075.1). Recommended concentration: 0.03-1ug/ml. Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Pax6
Long Name
Paired Box Gene 6
Alternate Names
keratitis), MGC17209, Oculorhombin, paired box 6, paired box protein Pax-6
Gene Symbol
PAX6
UniProt
Additional Pax6 Products
Product Documents for Pax6 Antibody
Product Specific Notices for Pax6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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