Paxillin [p Tyr118] Antibody (2E6P0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16929

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16929-100ul
NBP3-16929-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2E6P0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Paxillin Antibodies »

Product Summary for Paxillin [p Tyr118] Antibody (2E6P0)

Immunogen

A synthetic phosphorylated peptide around Y118 of human Paxillin (P49023). HVYSF

Modification

p Tyr 118

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Paxillin [p Tyr118] Antibody (2E6P0)

Western Blot: Paxillin [p Tyr118] Antibody (2E6P0) [NBP3-16929] - Western blot analysis of extracts of various cell lines, using Paxillin Rabbit mAb (NBP3-16929) at 1:1000 dilution.A-431 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by pervanadate (1 mM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Paxillin [p Tyr118] Antibody (2E6P0)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Paxillin

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Alternate Names

PXN

Gene Symbol

PXN

Additional Paxillin Products

Product Documents for Paxillin [p Tyr118] Antibody (2E6P0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Paxillin [p Tyr118] Antibody (2E6P0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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