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Paxillin Antibody (LD2 sAB) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09004

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-09004-0.025mg
NBP3-09004-0.2mg

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # LD2 sAB

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Peptide corresponding to paxillin-LD2 motif (residues 141-156) fused to MBP; peptide sequence: NLSELDRLLLELNAVQHNP.

Specificity

The antibody binds specifically to the LD-2 motif of human paxillin with a Kd of 24 nM.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for Paxillin Antibody (LD2 sAB) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.

This antibody binds to the LD2-motif of human paxillin, a signal transduction adaptor protein involved in focal adhesion, with binding partners including integrins, Src, FAK or actin complexes. The antibody inhibits complex formation between paxillin and FAK. Paxillin is involved in both inside-out cell signalling and outside-to-inside cell signalling.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Paxillin

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Alternate Names

PXN

Gene Symbol

PXN

Additional Paxillin Products

Product Documents for Paxillin Antibody (LD2 sAB) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Paxillin Antibody (LD2 sAB) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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