PBEF/Visfatin/NAMPT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46755

Novus Biologicals, part of Bio-Techne
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NBP2-46755
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PBEF/Visfatin Antibodies »

Product Summary for PBEF/Visfatin/NAMPT Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PBEF/Visfatin/NAMPT Antibody

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-46755]

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-46755]

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-46755] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251 MG. Lane 4: Human plasma Lane 5: Human Liver tissue. Lane 6: Human Tonsil tissue
Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody [NBP2-46755]

Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody [NBP2-46755]

Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody [NBP2-46755] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Applications for PBEF/Visfatin/NAMPT Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBEF/Visfatin

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Entrez Gene IDs

10135 (Human)

Gene Symbol

NAMPT

UniProt

P43490

Additional PBEF/Visfatin Products

Product Documents for PBEF/Visfatin/NAMPT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PBEF/Visfatin/NAMPT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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