PBR Antibody (4I9K6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16497
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4I9K6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 70-169 of human PBR (P30536). ELGGFTEKAVVPLGLYTGQLALNWAWPPIFFGARQMGWALVDLLLVSGAAAATTVAWYQVSPLAARLLYPYLAWLAFTTTLNYCVWRDNHGWRGGRRLPE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PBR Antibody (4I9K6)
Western Blot: PBR Antibody (4I9K6) [NBP3-16497]
Western Blot: PBR Antibody (4I9K6) [NBP3-16497] - Western blot analysis of extracts of various cell lines, using PBR Rabbit mAb (NBP3-16497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: PBR Antibody (4I9K6) [NBP3-16497]
Immunocytochemistry/Immunofluorescence: PBR Antibody (4I9K6) [NBP3-16497] - Immunofluorescence analysis of U-2 OS cells using PBRRabbit mAb (NBP3-16497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: PBR Antibody (4I9K6) [NBP3-16497]
Immunohistochemistry-Paraffin: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry of paraffin-embedded human colon using PBRRabbit mAb (NBP3-16497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for PBR Antibody (4I9K6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PBR
Long Name
Peripheral-type Benzodiazepine Receptor
Alternate Names
TSPO
Gene Symbol
TSPO
Additional PBR Products
Product Documents for PBR Antibody (4I9K6)
Product Specific Notices for PBR Antibody (4I9K6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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