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PBR Antibody (4I9K6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16497

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16497-100ul
NBP3-16497-20ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4I9K6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 70-169 of human PBR (P30536). ELGGFTEKAVVPLGLYTGQLALNWAWPPIFFGARQMGWALVDLLLVSGAAAATTVAWYQVSPLAARLLYPYLAWLAFTTTLNYCVWRDNHGWRGGRRLPE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PBR Antibody (4I9K6)

Western Blot: PBR Antibody (4I9K6) [NBP3-16497]

Western Blot: PBR Antibody (4I9K6) [NBP3-16497]

Western Blot: PBR Antibody (4I9K6) [NBP3-16497] - Western blot analysis of extracts of various cell lines, using PBR Rabbit mAb (NBP3-16497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: PBR Antibody (4I9K6) [NBP3-16497]

Immunocytochemistry/ Immunofluorescence: PBR Antibody (4I9K6) [NBP3-16497]

Immunocytochemistry/Immunofluorescence: PBR Antibody (4I9K6) [NBP3-16497] - Immunofluorescence analysis of U-2 OS cells using PBRRabbit mAb (NBP3-16497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PBR Antibody (4I9K6) [NBP3-16497]

Immunohistochemistry-Paraffin: PBR Antibody (4I9K6) [NBP3-16497]

Immunohistochemistry-Paraffin: PBR Antibody (4I9K6) [NBP3-16497] - Immunohistochemistry of paraffin-embedded human colon using PBRRabbit mAb (NBP3-16497) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for PBR Antibody (4I9K6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PBR

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Peripheral-type Benzodiazepine Receptor

Alternate Names

TSPO

Gene Symbol

TSPO

Additional PBR Products

Product Documents for PBR Antibody (4I9K6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PBR Antibody (4I9K6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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