PBR Antibody (CL13359)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18560

Novus Biologicals, part of Bio-Techne
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NBP3-18560-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13359

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PBR Antibodies »

Product Specifications

Immunogen

This antibody was developed against a sythetic peptide for P30536. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PBR Antibody (CL13359)

Western Blot: PBR Antibody (CL13359) [NBP3-18560]

Western Blot: PBR Antibody (CL13359) [NBP3-18560]

Western Blot: PBR Antibody (CL13359) [NBP3-18560] - Analysis in human cell line PC-3 and human cell line CACO-2.
Immunocytochemistry/ Immunofluorescence: PBR Antibody (CL13359) [NBP3-18560]

Immunocytochemistry/ Immunofluorescence: PBR Antibody (CL13359) [NBP3-18560]

Immunocytochemistry/Immunofluorescence: PBR Antibody (CL13359) [NBP3-18560] - Staining of A-431 cells using the Anti-TSPO monoclonal antibody, showing specific staining in mitochondria and vesicles in green. Nuclear probes are is visualized in blue (where available).
Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560]

Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560]

Immunohistochemistry-Paraffin: PBR Antibody (CL13359) [NBP3-18560] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.
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Applications for PBR Antibody (CL13359)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PBR

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Peripheral-type Benzodiazepine Receptor

Alternate Names

TSPO

Gene Symbol

TSPO

Additional PBR Products

Product Documents for PBR Antibody (CL13359)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PBR Antibody (CL13359)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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