PBR Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-41398
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RDNHGWRGGRRLPE corresponding to C-Terminus according to NP_000705.2.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:33725191).
Specificity
This antibody is expected to recognise one isoform (NP_000705.2) only.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
18.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PBR Antibody
Western Blot: PBR Antibody [NB100-41398]
Western Blot: PBR Antibody [NB100-41398] - Staining of A431 cell lysate.(35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.Immunocytochemistry/ Immunofluorescence: PBR Antibody [NB100-41398]
Immunocytochemistry/Immunofluorescence: PBR Antibody [NB100-41398] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunohistochemistry: PBR Antibody [NB100-41398]
Immunohistochemistry: PBR Antibody [NB100-41398] - Correlative TSPO immunohistochemistry in a primary human grade III astrocytoma xenotransplant, Tumor + White Matter Tract (40X). Image collected and cropped by CiteAb from the following publication (www.journals.plos.org/plosone/article?id=10.1371/journal.pone.0141659) licensed under a CC-BY license.Applications for PBR Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Immunohistochemistry
2-4 ug/ml
Immunohistochemistry-Paraffin
2-4 ug/ml
Peptide ELISA
detection limit 1:128000
Western Blot
0.1-0.3 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PBR
Long Name
Peripheral-type Benzodiazepine Receptor
Alternate Names
TSPO
Entrez Gene IDs
706 (Human)
Gene Symbol
TSPO
UniProt
Additional PBR Products
Product Documents for PBR Antibody
Product Specific Notices for PBR Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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