PBX1 Antibody (4A2)

Novus Biologicals, part of Bio-Techne | Catalog # H00005087-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PBX1 Antibodies »

Product Specifications

Immunogen

PBX1 (NP_002576, 213 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEILNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEANIYAAKTAVTATNVSAHGS

Specificity

PBX1 - pre-B-cell leukemia transcription factor 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PBX1 Antibody (4A2)

Western Blot: PBX1 Antibody (4A2) [H00005087-M01]

Western Blot: PBX1 Antibody (4A2) [H00005087-M01]

Western Blot: PBX1 Antibody (4A2) [H00005087-M01] - PBX1 monoclonal antibody (M01), clone 4A2 Analysis of PBX1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01]

Immunocytochemistry/ Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01]

Immunocytochemistry/Immunofluorescence: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01]

Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01]

Immunohistochemistry-Paraffin: PBX1 Antibody (4A2) [H00005087-M01] - Analysis of monoclonal antibody to PBX1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
View 1 more images

Applications for PBX1 Antibody (4A2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 26610607).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PBX1

PBX1 binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development a

Alternate Names

DKFZp686B09108, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, Pre-B cell leukemia transcription factor-1, pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, PRL

Entrez Gene IDs

5087 (Human)

Gene Symbol

PBX1

OMIM

176310 (Human)

UniProt

NP_002576 (Human)

Additional PBX1 Products

Product Documents for PBX1 Antibody (4A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PBX1 Antibody (4A2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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