PBX1 Antibody (OTI1C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00872

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C11

Concentration

0.29 mg/ml

View all PBX1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PBX1(NP_002576) produced in HEK293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PBX1 Antibody (OTI1C11)

Western Blot: PBX1 Antibody (OTI1C11) [NBP2-00872]

Western Blot: PBX1 Antibody (OTI1C11) [NBP2-00872]

Western Blot: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PBX1.
Immunocytochemistry/Immunofluorescence: PBX1 Antibody (1C11) [NBP2-00872] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1.
Immunohistochemistry-Paraffin: PBX1 Antibody (OTI1C11) [NBP2-00872]

Immunohistochemistry-Paraffin: PBX1 Antibody (OTI1C11) [NBP2-00872]

Immunohistochemistry-Paraffin: PBX1 Antibody (1C11) [NBP2-00872] - Staining of paraffin-embedded Human liver tissue using anti-PBX1 mouse monoclonal antibody.
View 1 more images

Applications for PBX1 Antibody (OTI1C11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.29 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PBX1

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Alternate Names

DKFZp686B09108, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, Pre-B cell leukemia transcription factor-1, pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, PRL

Entrez Gene IDs

5087 (Human)

Gene Symbol

PBX1

Additional PBX1 Products

Product Documents for PBX1 Antibody (OTI1C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PBX1 Antibody (OTI1C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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