PBX1 Antibody (PCRP-PBX1-3C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13991

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-PBX1-3C8

Concentration

0.2 mg/ml

View all PBX1 Antibodies »

Product Summary for PBX1 Antibody (PCRP-PBX1-3C8)

Immunogen

Recombinant full-length human PBX1 protein (Uniprot: P40424)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

86.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14035)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PBX1 Antibody (PCRP-PBX1-3C8)

Immunocytochemistry/ Immunofluorescence: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991]

Immunocytochemistry/ Immunofluorescence: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991]

Immunocytochemistry/Immunofluorescence: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using PBX1 Antibody (PCRP-PBX1-3C8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991]

Flow Cytometry: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991]

Flow Cytometry: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991] - Flow cytometric analysis of PFA-fixed HeLa cells. PBX1 antibody (PCRP-PBX1-3C8) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991]

Protein Array: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991]

Protein Array: PBX1 Antibody (PCRP-PBX1-3C8) [NBP3-13991] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PBX1 Antibody (PCRP-PBX1-3C8).

Applications for PBX1 Antibody (PCRP-PBX1-3C8)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PBX1

PBX1 binds the sequence 5'-ATCAATCAA-3'. Acts as a transcriptional activator of PF4 in complex with MEIS1. Converted into a potent transcriptional activator by the (1;19) translocation. May have a role in steroidogenesis and, subsequently, sexual development a

Alternate Names

DKFZp686B09108, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, Pre-B cell leukemia transcription factor-1, pre-B-cell leukemia homeobox 1, pre-B-cell leukemia transcription factor 1, PRL

Gene Symbol

PBX1

UniProt

P40424 (Human)

Additional PBX1 Products

Product Documents for PBX1 Antibody (PCRP-PBX1-3C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PBX1 Antibody (PCRP-PBX1-3C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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