PBX1 Antibody (PSH0-24)

Western Blot: PBX1 Antibody (PSH0-24) [NBP3-32720]

Western Blot: PBX1 Antibody (PSH0-24) [NBP3-32720] - Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (NBP3-32720) at 1/1,000 dilution.

Lane 1: Hela cell lysate (20 ug/Lane)
Lane 2: HEK-293 cell lysate (20 ug/Lane)
Lane 3: MCF-7 cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: A431 cell lysate (20 ug/Lane)
Lane 6: NIH/3T3 cell lysate (20 ug/Lane)
Lane 7: Mouse testis tissue lysate (40 ug/Lane)
Lane 8: Rat pancreas tissue lysate (40 ug/Lane)

Predicted band size: 47 kDa
Observed band size: 40/47 kDa

Exposure time: 3 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32720) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PBX1 Antibody (PSH0-24) [NBP3-32720]

Immunohistochemistry: PBX1 Antibody (PSH0-24) [NBP3-32720] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PBX1 antibody (NBP3-32720) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32720) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.