PCBP1 Antibody (OTI3D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46336

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D12

Concentration

1 mg/ml

View all PCBP1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-225 of human PCBP1(NP_006187) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCBP1 Antibody (OTI3D12)

Western Blot: PCBP1 Antibody (OTI3D12) [NBP2-46336]

Western Blot: PCBP1 Antibody (OTI3D12) [NBP2-46336]

Western Blot: PCBP1 Antibody (3D12) [NBP2-46336] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCBP1.
Immunohistochemistry: PCBP1 Antibody (OTI3D12) [NBP2-46336]

Immunohistochemistry: PCBP1 Antibody (OTI3D12) [NBP2-46336]

Immunohistochemistry: PCBP1 Antibody (3D12) [NBP2-46336] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: PCBP1 Antibody (OTI3D12) [NBP2-46336]

Western Blot: PCBP1 Antibody (OTI3D12) [NBP2-46336]

Western Blot: PCBP1 Antibody (3D12) [NBP2-46336] - Analysis of extracts (10ug) from 5 different cell lines and 4 human tissue by using PCBP1 monoclonal antibody (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)at 1:200 dilution.
View 5 more images

Applications for PCBP1 Antibody (OTI3D12)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PCBP1

PCBP1 is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq]

Alternate Names

alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, hnRNP E1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, nucleic acid binding protein sub 2.3, Nucleic acid-binding protein SUB2.3, poly(rC) binding protein 1, poly(rC)-binding protein 1

Gene Symbol

PCBP1

Additional PCBP1 Products

Product Documents for PCBP1 Antibody (OTI3D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PCBP1 Antibody (OTI3D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.