PCBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52114

Novus Biologicals, part of Bio-Techne
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NBP1-52114
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PCBP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ADLAKLNQVARQQSH corresponding to internal region according to CAA55016.1.

Epitope

C-ADLAKLNQVARQQSH

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PCBP1 Antibody

Western Blot: PCBP1 Antibody [NBP1-52114]

Western Blot: PCBP1 Antibody [NBP1-52114]

Western Blot: PCBP1 Antibody [NBP1-52114] - Analysis of PCBP1 (aa 234-247) in HeLa lysate (35 ug protein in RIPA buffer) using NBP1-52114 at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-52114]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-52114]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-52114] - Staining of human lung. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PCBP1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 38 kDa band observed in lysates of HeLa and in human placenta lysates (calculated MW of 37.5 kDa band according to NP_006187.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PCBP1

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq]

Alternate Names

alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, hnRNP E1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, nucleic acid binding protein sub 2.3, Nucleic acid-binding protein SUB2.3, poly(rC) binding protein 1, poly(rC)-binding protein 1

Entrez Gene IDs

5093 (Human); 23983 (Mouse); 500242 (Rat)

Gene Symbol

PCBP1

UniProt

NP_006187.2 (Human)

Additional PCBP1 Products

Product Documents for PCBP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PCBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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