PCCA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13736

Novus Biologicals, part of Bio-Techne
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NBP2-13736
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCCA Antibodies »

Product Summary for PCCA Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: YPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCCA Antibody

Western Blot: PCCA Antibody [NBP2-13736]

Western Blot: PCCA Antibody [NBP2-13736]

Western Blot: PCCA Antibody [NBP2-13736] - Analysis in human cell line HepG2.
Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-13736]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-13736]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-13736] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-13736]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-13736]

Immunohistochemistry-Paraffin: PCCA Antibody [NBP2-13736] - Analysis in human kidney and tonsil tissues. Corresponding PCCA RNA-seq data are presented for the same tissues.
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Applications for PCCA Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCCA

PCCA is encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

EC 6.4.1, EC 6.4.1.3, pccA complementation group, PCCase alpha subunit, PCCase subunit alpha, propanoyl-CoA:carbon dioxide ligase alpha subunit, Propanoyl-CoA:carbon dioxide ligase subunit alpha, propionyl CoA carboxylase, alpha polypeptide, propionyl Coenzyme A carboxylase, alpha polypeptide, propionyl-CoA carboxylase alpha chain, mitochondrial

Gene Symbol

PCCA

Additional PCCA Products

Product Documents for PCCA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PCCA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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