PCIF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-37957
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PCIF1 (NP_071387.1).
Sequence:
MANENHGSPREEASLLSHSPGTSNQSQPCSPKPIRLVQDLPEELVHAGWEKCWSRRENRPYYFNRFTNQSLWEMPVLGQHDVISDPLGLNATPLPQDSSLVETPPAENKPRKRQLSEEQPSGNGVKKPKIEIPVTPTGQSVPSSPSIPGTPTLKMWGTSPEDKQQAALLRPTEVYWDLDIQTNAVIKHRGPSEVLPPHPE
Sequence:
MANENHGSPREEASLLSHSPGTSNQSQPCSPKPIRLVQDLPEELVHAGWEKCWSRRENRPYYFNRFTNQSLWEMPVLGQHDVISDPLGLNATPLPQDSSLVETPPAENKPRKRQLSEEQPSGNGVKKPKIEIPVTPTGQSVPSSPSIPGTPTLKMWGTSPEDKQQAALLRPTEVYWDLDIQTNAVIKHRGPSEVLPPHPE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PCIF1 Antibody
Immunohistochemistry: PCIF1 Antibody [NBP3-37957] -
Immunohistochemistry: PCIF1 Antibody [NBP3-37957] - Immunohistochemistry analysis of paraffin-embedded Rat brain using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Western Blot: PCIF1 Antibody [NBP3-37957] -
Western Blot: PCIF1 Antibody [NBP3-37957] - Western blot analysis of various lysates using PCIF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry: PCIF1 Antibody [NBP3-37957] -
Immunohistochemistry: PCIF1 Antibody [NBP3-37957] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using PCIF1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.Applications for PCIF1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50 - 1:100
Immunoprecipitation
0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PCIF1
Alternate Names
bA465L10.1, C20orf67, chromosome 20 open reading frame 67, PDX-1 C terminus-interacting factor 1, PDX1 C-terminal inhibiting factor 1, phosphorylated CTD-interacting factor 1
Gene Symbol
PCIF1
Additional PCIF1 Products
Product Documents for PCIF1 Antibody
Product Specific Notices for PCIF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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