PCK1 Antibody (3E4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005105-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCK1 Antibodies »

Product Specifications

Immunogen

PCK1 (AAH23978, 1 a.a. ~ 622 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTRMGTPVLEALGDGEFVKCLHSVGCPLPLQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRMASRLAKEEGWLAEHMLVLGITNPEGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM

Localization

Cytoplasmic

Specificity

PCK1 - phosphoenolpyruvate carboxykinase 1 (soluble)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PCK1 Antibody (3E4)

Western Blot: PCK1 Antibody (3E4) [H00005105-M01]

Western Blot: PCK1 Antibody (3E4) [H00005105-M01]

Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01]

Immunocytochemistry/ Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01]

Immunocytochemistry/Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of monoclonal antibody to PCK1 on HepG2 cell. Antibody concentration 10 ug/ml.
Western Blot: PCK1 Antibody (3E4) [H00005105-M01]

Western Blot: PCK1 Antibody (3E4) [H00005105-M01]

Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - PCK1 monoclonal antibody (M01), clone 3E4 Analysis of PCK1 expression in HepG2.
View 1 more images

Applications for PCK1 Antibody (3E4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCK1

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.

Long Name

Phosphoenolpyruvate Carboxykinase 1

Alternate Names

PEPCK-C, PEPCK1

Entrez Gene IDs

5105 (Human)

Gene Symbol

PCK1

OMIM

261680 (Human)

UniProt

AAH23978 (Human)

Additional PCK1 Products

Product Documents for PCK1 Antibody (3E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PCK1 Antibody (3E4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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