PCK1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80928
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSM
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PCK1 Antibody
![Western Blot: PCK1 Antibody [NBP1-80928]](https://resources.bio-techne.com/images/products/PCK1-Antibody-Western-Blot-NBP1-80928-img0005.jpg "Western Blot: PCK1 Antibody [NBP1-80928]")
Western Blot: PCK1 Antibody [NBP1-80928]
Western Blot: PCK1 Antibody [NBP1-80928] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp![Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80928]](https://resources.bio-techne.com/images/products/PCK1-Antibody-Immunohistochemistry-Paraffin-NBP1-80928-img0002.jpg "Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80928]")
Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80928]
Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80928] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.Applications for PCK1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PCK1
Long Name
Phosphoenolpyruvate Carboxykinase 1
Alternate Names
PEPCK-C, PEPCK1
Entrez Gene IDs
5105 (Human)
Gene Symbol
PCK1
UniProt
Additional PCK1 Products
Product Documents for PCK1 Antibody
Product Specific Notices for PCK1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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