PCMT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97711

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PCMT1 Antibodies »

Product Summary for PCMT1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human PCMT1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PCMT1 Antibody

Western Blot: PCMT1 Antibody [NBP2-97711]

Western Blot: PCMT1 Antibody [NBP2-97711]

Western Blot: PCMT1 Antibody [NBP2-97711] - Anti-PCMT1 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 28 kDa
Immunohistochemistry-Paraffin: PCMT1 Antibody [NBP2-97711]

Immunohistochemistry-Paraffin: PCMT1 Antibody [NBP2-97711]

Immunohistochemistry-Paraffin: PCMT1 Antibody [NBP2-97711] - Immunochemical staining of human PCMT1 in human stomach with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.

Applications for PCMT1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCMT1

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]

Alternate Names

EC 2.1.1, EC 2.1.1.77, L-isoaspartyl protein carboxyl methyltransferase, L-isoaspartyl/D-aspartyl methyltransferase, PIMT, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, protein-L-isoaspartate (D-aspartate) O-methyltransferase, protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene Symbol

PCMT1

Additional PCMT1 Products

Product Documents for PCMT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCMT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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