PCNA associated factor Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80555
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptide directed towards the N terminal of human KIAA0101. Peptide sequence MVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKEHVLCNLI. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for PCNA associated factor Antibody
![Western Blot: PCNA associated factor Antibody [NBP1-80555]](https://resources.bio-techne.com/images/products/PCNA-associated-factor-Antibody-Western-Blot-NBP1-80555-img0003.jpg "Western Blot: PCNA associated factor Antibody [NBP1-80555]")
Western Blot: PCNA associated factor Antibody [NBP1-80555]
Western Blot: PCNA associated factor Antibody [NBP1-80555] - KIAA0101 tv2 is highly expressed in adjacent non-tumorous liver tissues (NTs) compared to hepatocellular carcinoma (HCC) tissues(A-B) KIAA0101 tv2 protein levels were analyzed by western blot in 42 pairs of HCC cases and NTs. The graph shows the mean+-standard error of mean (SEM) of KIAA0101 tv2 protein overexpression rates by western blot. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/16702) licensed under a CC-BY license.![Western Blot: PCNA associated factor Antibody [NBP1-80555]](https://resources.bio-techne.com/images/products/PCNA-associated-factor-Antibody-Western-Blot-NBP1-80555-img0002.jpg "Western Blot: PCNA associated factor Antibody [NBP1-80555]")
Western Blot: PCNA associated factor Antibody [NBP1-80555]
Western Blot: PCNA associated factor Antibody [NBP1-80555] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP1-80555] -
Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP1-80555] - KIAA0101 tv2 is highly expressed in adjacent non-tumorous liver tissues (NTs) compared to hepatocellular carcinoma (HCC) tissues(A-B) KIAA0101 tv2 protein levels were analyzed by western blot in 42 pairs of HCC cases & NTs. The graph shows the mean±standard error of mean (SEM) of KIAA0101 tv2 protein overexpression rates by western blot. (C-D) All tissues were assessed by immunohistochemistry (IHC) using anti-KIAA0101 tv2 polyclonal antibody. Representative images are shown (Original magnification ×400). Each bar represents results from three independent experiments. ***P<0.005. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28410205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PCNA associated factor Antibody
Application
Recommended Usage
Western Blot
1.0 ug/ml
Application Notes
Use in Immunohistochemistry paraffin reported in scientific literature (PMID 28410205).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PCNA associated factor
Alternate Names
HCV NS5A-transactivated protein 9, Hepatitis C virus NS5A-transactivated protein 9, KIAA0101, NS5ATP9p15PAF, OEATC-1OEATC1, Overexpressed in anaplastic thyroid carcinoma 1, p15(PAF), PAF, PCNA-associated factor
Gene Symbol
PCLAF
UniProt
Additional PCNA associated factor Products
Product Documents for PCNA associated factor Antibody
Product Specific Notices for PCNA associated factor Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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