PCNA associated factor Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32112
![Western Blot: PCNA associated factor Antibody [NBP2-32112]](https://resources.bio-techne.com/images/products/PCNA-associated-factor-Antibody-Western-Blot-NBP2-32112-img0005.jpg "Western Blot: PCNA associated factor Antibody [NBP2-32112]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for PCNA associated factor Antibody
Immunogen
The immunogen this antibody was made to, maps to a region between residue 61 to 111 of human PCNA-Associated Factor using the numbering given in entry NP_055551.1 (GeneID 9768).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PCNA associated factor Antibody
Western Blot: PCNA associated factor Antibody [NBP2-32112]
Western Blot: PCNA associated factor Antibody [NBP2-32112] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PAF antibody NBP2-32112 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112]](https://resources.bio-techne.com/images/products/PCNA-associated-factor-Antibody-Immunohistochemistry-NBP2-32112-img0002.jpg "Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112]")
Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112]
Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PAF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).Applications for PCNA associated factor Antibody
Application
Recommended Usage
Immunohistochemistry
1:2000 - 1:10000
Immunohistochemistry-Paraffin
1:2000 - 1:10000
Western Blot
1:2000 - 1:10000
Application Notes
Immunoprecipitation Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PCNA associated factor
Alternate Names
HCV NS5A-transactivated protein 9, Hepatitis C virus NS5A-transactivated protein 9, KIAA0101, NS5ATP9p15PAF, OEATC-1OEATC1, Overexpressed in anaplastic thyroid carcinoma 1, p15(PAF), PAF, PCNA-associated factor
Gene Symbol
PCLAF
UniProt
Additional PCNA associated factor Products
Product Documents for PCNA associated factor Antibody
Product Specific Notices for PCNA associated factor Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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