PCNP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86312
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for PCNP Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NEDEDSEPEEMPPEAKMRMKNIGRDTPTSAGPNSFNKGKHGFSDNQKLWERNIKSHLGNVHDQD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PCNP Antibody
Western Blot: PCNP Antibody [NBP1-86312]
Western Blot: PCNP Antibody [NBP1-86312] - Analysis in control (vector only transfected HEK293T lysate) and PCNP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: PCNP Antibody [NBP1-86312]
Immunocytochemistry/Immunofluorescence: PCNP Antibody [NBP1-86312] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.Immunohistochemistry-Paraffin: PCNP Antibody [NBP1-86312]
Immunohistochemistry-Paraffin: PCNP Antibody [NBP1-86312] - Staining of human colon shows strong nuclear positivity in glandular cells.Applications for PCNP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PCNP
Alternate Names
DKFZp781I24156, PEST proteolytic signal containing nuclear protein, PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein
Gene Symbol
PCNP
Additional PCNP Products
Product Documents for PCNP Antibody
Product Specific Notices for PCNP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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