Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PCNP Antibodies »

Product Summary for PCNP Antibody

Immunogen

This antibody maps to a region between residue 1 to 50 of human PEST Proteolytic Signal Containing Nuclear Protein using the numbering given in entry NP_065090.1 (GeneID 57092).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNP Antibody

Western Blot: PCNP Antibody [NBP2-37692]

Western Blot: PCNP Antibody [NBP2-37692]

Western Blot: PCNP Antibody [NBP2-37692] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PCNP Antibody [NBP2-37692]

Immunoprecipitation: PCNP Antibody [NBP2-37692]

Immunoprecipitation: PCNP Antibody [NBP2-37692] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for IP at 6 ug per reaction. PCNP was also immunoprecipitated by rabbit anti-PCNP antibody. For blotting immunoprecipitated PCNP, was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PCNP Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:10000 - 1:25000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCNP

PCNP may be involved in cell cycle regulation

Alternate Names

DKFZp781I24156, PEST proteolytic signal containing nuclear protein, PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

Gene Symbol

PCNP

UniProt

NP_065090.1 (Human)

Additional PCNP Products

Product Documents for PCNP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PCNP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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