PCPE-1/PCOLCE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55270

Novus Biologicals, part of Bio-Techne
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NBP2-55270
NBP2-55270-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PCPE-1 Antibodies »

Product Summary for PCPE-1/PCOLCE Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PKSQPPEKTEESPSAPDAPTCPKQCRRTGTLQSNFCASSLVVTATVKSMVREPGEGLAVTVSLIGAYKTGGLDLPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCPE-1/PCOLCE Antibody

Western Blot: PCPE-1/PCOLCE Antibody [NBP2-55270]

Western Blot: PCPE-1/PCOLCE Antibody [NBP2-55270]

Western Blot: PCPE-1/PCOLCE Antibody [NBP2-55270] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: PCPE-1/PCOLCE Antibody [NBP2-55270]

Immunocytochemistry/ Immunofluorescence: PCPE-1/PCOLCE Antibody [NBP2-55270]

Immunocytochemistry/Immunofluorescence: PCPE-1/PCOLCE Antibody [NBP2-55270] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.

Applications for PCPE-1/PCOLCE Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCPE-1

Collagen maturation includes the processing of N- and C-propeptides by Procollagen N- and C-proteinases encoded by the ADAMTS2 and BMP-1 genes, respectively. PCPE (Procollagen C-proteinase Enhancer) binds to either side of the C-propeptide cleavage site, thereby facilitating the Procollagen C-proteinase activity of BMP1. PCPE does not enhance the chordinase activity of BMP-1.

Long Name

Procollagen C-Proteinase Enhancer-1

Alternate Names

PCOLCE, PCPE1

Gene Symbol

PCOLCE

Additional PCPE-1 Products

Product Documents for PCPE-1/PCOLCE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PCPE-1/PCOLCE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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