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PDCL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80626

Novus Biologicals, part of Bio-Techne
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NBP1-80626
NBP1-80626-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EWNDILRKKGILPPKESLKELEEEAEEEQRILQQSVVKTYEDMTLEELEDHEDEFNEEDERAIEMYRRRRLAEWKATKLK

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26059764).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDCL3 Antibody

Western Blot: PDCL3 Antibody [NBP1-80626]

Western Blot: PDCL3 Antibody [NBP1-80626]

Western Blot: PDCL3 Antibody [NBP1-80626] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: PDCL3 Antibody [NBP1-80626]

Immunocytochemistry/ Immunofluorescence: PDCL3 Antibody [NBP1-80626]

Immunocytochemistry/Immunofluorescence: PDCL3 Antibody [NBP1-80626] - Staining of human cell line U-2 OS shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDCL3 Antibody [NBP1-80626]

Immunohistochemistry-Paraffin: PDCL3 Antibody [NBP1-80626]

Immunohistochemistry-Paraffin: PDCL3 Antibody [NBP1-80626] - Staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.

Applications for PDCL3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF reported in scientific literature (PMID: 26059764). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDCL3

The PDCL3 gene encodes a member of the phosducin-like protein family and is a putative modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin. Members of the phosducin-like protein family have been show

Alternate Names

HTPHLP, IAP-associated factor VIAF1, MGC3062, PHLP3, phosducin-like 3, phosducin-like protein 3, PhPL3, VIAF, VIAF-1, VIAF1PHLP2A, Viral IAP-associated factor 1

Entrez Gene IDs

79031 (Human)

Gene Symbol

PDCL3

UniProt

Additional PDCL3 Products

Product Documents for PDCL3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDCL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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