PDE1B Antibody (OTI6E4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73278

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI6E4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PDE1B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE1B Antibody (OTI6E4) - Azide and BSA Free

Western Blot: PDE1B Antibody (OTI6E4)Azide and BSA Free [NBP2-73278]

Western Blot: PDE1B Antibody (OTI6E4)Azide and BSA Free [NBP2-73278]

Western Blot: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B.
Immunocytochemistry/ Immunofluorescence: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278]

Immunocytochemistry/ Immunofluorescence: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278]

Immunocytochemistry/Immunofluorescence: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B.
Immunohistochemistry: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278]

Immunohistochemistry: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278]

Immunohistochemistry: PDE1B Antibody (OTI6E4) - Azide and BSA Free [NBP2-73278] - Staining of paraffin-embedded Human Kidney tissue using anti-PDE1B mouse monoclonal antibody.
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Applications for PDE1B Antibody (OTI6E4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE1B

PDE1B, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The tissue distribution of PDE1B is different from other PDE1 gene family members. In testis PDE1B is expressed uniformly through out the seminiferous epithelium and in the interstistium in contrast to PDE1A and PDE1C that is expressed in elongated to mature spermatids.

Long Name

Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Alternate Names

63 kDa Cam-PDE, Cam-PDE 1B, PDES1B

Gene Symbol

PDE1B

Additional PDE1B Products

Product Documents for PDE1B Antibody (OTI6E4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PDE1B Antibody (OTI6E4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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