PDE1C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-672

Novus Biologicals, part of Bio-Techne
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NB300-672
NB300-672-0.025mg
NB300-672-0.05mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.75 mg/ml

View all PDE1C Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on PDE1C gene.

Specificity

The does not cross react with PDE1A, PDE1B or other PDEs.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PDE1C Antibody

Western Blot: PDE1C Antibody [NB300-672]

Western Blot: PDE1C Antibody [NB300-672]

Western Blot: PDE1C Antibody [NB300-672] - Rat brain on SDS page. Aproximately 50-60 ug total rat brain homogenate/lane. 1:250 antibody dilution in DiluOBuffer. Lane 1. Rabbit, Lane 2: Rabbit
Immunohistochemistry: PDE1C Antibody [NB300-672]

Immunohistochemistry: PDE1C Antibody [NB300-672]

Immunohistochemistry: PDE1C Antibody [NB300-672] - Human ovarian medulla tissue. 1:1500 antibody dilution
Western Blot: PDE1C Antibody [NB300-672]

Western Blot: PDE1C Antibody [NB300-672]

Western Blot: PDE1C Antibody [NB300-672] - 1:500 antibody dilution in DiluOBuffer.

Applications for PDE1C Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:40-1:100

Immunohistochemistry-Paraffin

1:40-1:100

Immunoprecipitation

1:200

Western Blot

1:250-1:500
Application Notes
Immunoprecipitation and Western Blot: This antibody labels multiple bands in the 70-87 kDa range all believed to be various PDE1C variants. Optimal dilutions and concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.75 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDE1C

PDE1C is a phosphodiesterase activated by the binding of calmodulin in the presence of calcium. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodiesterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1C gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. The tissue distribution of PDE1C is different from other PDE1 gene family members.

Long Name

Dual specificity Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C

Alternate Names

Cam-PDE 1C, Hcam3

Entrez Gene IDs

18575 (Mouse); 81742 (Rat)

Gene Symbol

PDE1C

Additional PDE1C Products

Product Documents for PDE1C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDE1C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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