PDE3B Antibody (SMCP3B) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43333

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # SMCP3B

Format

BSA Free

Concentration

0.5 mg/ml

View all PDE3B Antibodies »

Product Specifications

Immunogen

His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for PDE3B Antibody (SMCP3B) - BSA Free

Western Blot: PDE3B Antibody (SMCP3B)BSA Free [NBP1-43333]

Western Blot: PDE3B Antibody (SMCP3B)BSA Free [NBP1-43333]

Western Blot: PDE3B Antibody (SMCP3B) [NBP1-43333] - Lysates prepared from mouse heart (lane 1) or lymph node cells (lane 2) were run under reducing conditions on a 3-8% Tris-Acetate gel. The membrane was probed with 2 ug/mL of Anti-Mouse PDE3B Purified, followed by Anti-Rat IgG HRP.

Applications for PDE3B Antibody (SMCP3B) - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
This SMCP3B antibody has been tested by immunoblotting of mouse lymph node cells and mouse heart under reducing conditions. It can be used at 1-5 ug/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDE3B

Cyclic nucleotide phosphodiesterases (PDE) catalyze the hydrolysis of cAMP and cGMP thereby regulating the effects of these second messengers. There are several families of PDE which share common structural organization. The PDE3 family has two members, 3A and 3B that are products of two separate genes. PDE3B (~130 kDa) is expressed predominantly in adipose tissue and lymphocytes, while PDE3A (~120 kDa) is expressed predominantly in platelets and heart tissue. The monoclonal antibody SMCP3B reacts with mouse PDE3B. The SMCP3B antibody was generated against amino acids 643-1100 in the C-terminus and does not cross-react with mouse PDE3A, as determined by western blot analysis of lysates of mouse heart tissue. The SMCP3B antibody does not cross-react with human PDE3B.

Long Name

cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Alternate Names

CGI-PDE B, CGIP1, CGIPDE1

Gene Symbol

PDE3B

Additional PDE3B Products

Product Documents for PDE3B Antibody (SMCP3B) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDE3B Antibody (SMCP3B) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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